The mechanism of integration preference to heterochromatin of yeast retrotransposon Ty5
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چکیده
vii CHAPTER I. GENERAL INTRODUCTION 1 Transposable elements 1 Integrase and integration 3 Ty elements and targeted integration 6 Heterochromatin and silencing 9
منابع مشابه
Controlling integration specificity of a yeast retrotransposon.
Retrotransposons and retroviruses integrate nonrandomly into eukaryotic genomes. For the yeast retrotransposon Ty5, integration preferentially occurs within domains of heterochromatin. Targeting to these locations is determined by interactions between an amino acid sequence motif at the C terminus of Ty5 integrase (IN) called the targeting domain, and the heterochromatin protein Sir4p. Here we ...
متن کاملAccess to DNA establishes a secondary target site bias for the yeast retrotransposon Ty5.
Integration sites for many retrotransposons and retroviruses are determined by interactions between retroelement-encoded integrases and specific DNA-bound proteins. The Saccharomyces retrotransposon Ty5 preferentially integrates into heterochromatin because of interactions between Ty5 integrase and the heterochromatin protein silent information regulator 4. We mapped over 14,000 Ty5 insertions ...
متن کاملTagging chromatin with retrotransposons: target specificity of the Saccharomyces Ty5 retrotransposon changes with the chromosomal localization of Sir3p and Sir4p.
Retrotransposon and retroviral insertions are not randomly distributed on chromosomes, suggesting that retroelements actively select integration sites. This is the case for the yeast Ty5 retrotransposons, which preferentially integrate into domains of silent chromatin at the HM loci and telomeres. Here we demonstrate that loss of Sir3p or Sir4p-components of silent chromatin-causes a greater th...
متن کاملStudying the replication mechanism of the yeast retrotransposon Ty5 by molecular and computational approaches
vi CHAPTER I. GENERAL INTRODUCTION 1 Taxonomy of transposable elements 1 Retroviruses and LTR retrotransposons 2 Overview of reverse transcription 7 Retroelement proteins involved in reverse transcription 10 Diversity in priming LTR retroelements reverse transcription 12 Integration and recombination of cDNA 15 Evolution of retroelements 19 The Ty5 system 19 Dissertation organization 23 Referen...
متن کاملThe yeast retrotransposon Ty5 uses the anticodon stem-loop of the initiator methionine tRNA as a primer for reverse transcription.
Retrotransposons and retroviruses replicate by reverse transcription of an mRNA intermediate. Most retroelements initiate reverse transcription from a host-encoded tRNA primer. DNA synthesis typically extends from the 3'-OH of the acceptor stem, which is complementary to sequences on the retroelement mRNA (the primer binding site, PBS). However, for some retrotransposons, including the yeast Ty...
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تاریخ انتشار 2015